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Last reviewed July 10, 2026

Vitamin D3 (cholecalciferol)

Vitamin D3 is usually made from lanolin in sheep's wool, but a lichen-derived vegan D3 exists, and D2 is plant-based. Check the label or ask.

Where it hides

  • Supplements and multivitamins
  • Fortified plant milks and juices
  • Fortified cereals
  • Some yogurts and spreads

What it is

Vitamin D comes in two supplement forms, D2 and D3, and the animal question is all about how each is made. The NIH Office of Dietary Supplements spells out the default source of D3.

Primary source 01 / 03

vitamin D3 is typically produced with irradiation of 7-dehydrocholesterol from lanolin obtained from the wool of sheep

NIH Office of Dietary Supplements US government fact sheet Vitamin D: Fact Sheet for Health Professionals, 2024

Lanolin is the grease from sheep's wool, so standard D3 is animal-derived. The good news is that a vegan D3 now exists, and D2 sits outside the problem entirely because it is made from yeast or fungi.

Primary source 02 / 03

An animal-free version of vitamin D3 sourced from lichen is also available

NIH Office of Dietary Supplements US government fact sheet Vitamin D: Fact Sheet for Health Professionals, 2024

How to choose

For a fortified food or a supplement, the label rarely states the source of its D3, so a product carrying a vegan mark, a lichen-derived claim, or plainly listing D2 is the safe pick. When none of that is printed, the sourcing genuinely varies, and the fact sheet is blunt about the fix.

Primary source 03 / 03

People who avoid all animal-sourced products can contact dietary supplement manufacturers to ask about their sourcing and processing techniques.

NIH Office of Dietary Supplements US government fact sheet Vitamin D: Fact Sheet for Health Professionals, 2024

Frequently asked

Is vitamin D3 always from sheep?

No, but usually. Most D3 is made from lanolin, the grease in sheep's wool, so it is not vegan by default. A vegan D3 made from lichen is now available, and vitamin D2 is made from yeast or fungi and is vegan.

Should vegans take D2 or D3?

Either works to raise vitamin D, though D3 tends to raise blood levels a little more effectively. Vegans can get D3 from a lichen-derived supplement or use D2; our vitamin D page in The Evidence covers the trade-offs.

The sources

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